Human IL-12 (p70) ELISA MAX™ Deluxe 20 plates | Medical Supply Company

Human IL-12 (p70) ELISA MAX™ Deluxe 5 plates

SKU: 431704 Categories: Deluxe Set, ELISA, Human, Sandwich Tag: Biolegend BV

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Human IL-12 (p70) ELISA MAX™ Deluxe 5 plates

Cytokine detection

IL-12, IL-23 and IL-35 share common subunits, utilizing combinations of p40, p19 and p35 proteins. Caution must be used when selecting antibodies and assays when specific identification, measurement, as well as activation state discrimination, is required.

1. Active IL-12 consists of two subunits: p40 + p35.
2. p40 can also exist as a monomer (IL-12 p40) or a homodimer (IL-12 p80).
3. Besides contributing to IL-12, p40 is also found in IL-23, a heterodimer of p40 and p19.
4. Similarly, p35 not only contributes to IL-12, but is also found in the heterodimer IL-35 (p35 and EBI3).

Note that assays using antibodies specific for the p40 subunit will be unable to discriminate between the active IL-12, monomeric p40, dimeric p40, and IL-23; likewise, assays using p35 detection alone will pick up both IL-12 (heterodimer) and IL-35.

BioLegend ELISA assays

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend’s ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!
BioLegend’s ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Reactivity

Human

Application

ELISA

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Application References (PubMed link indicates BioLegend citation)

Peng JC, et al. 2006. J. Immunother. 29:606. PubMed
Chiesa, S., et al. 2011. PNAS. PubMed.
Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed
Chiesa S, et al. 2011. Proc Natl Acad Sci U S A. 108:17384. PubMed
Penafuerte C, et al. 2017. Oncoimmunology. 6:e1321185. PubMed

Sensitivity

4 pg/mL

Standard Range

15.6-1,000 pg/mL

Materials Not Included

A microplate reader capable of measuring absorbance at 450 nm
Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
Deionized (DI) water
PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na₂HPO₄, 0.2 g KH₂PO₄, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
Wash bottle or automated microplate washer
Stop Solution (e.g. 2N H₂SO₄) Biolegend Cat. No. 423001 is recommended
Log-Log graph paper or software for data analysis
Tubes to prepare standard dilutions
Timer
Plate Sealer
Absorbent paper

Kit Contents

Human IL-12 (p70) ELISA MAX™ Capture Antibody (200X)
Human IL-12 (p70) ELISA MAX™ Detection Antibody (200X)
Human IL-12 (p70) Standard
Avidin-HRP (1000X)
Substrate Solution A
Substrate Solution B
Coating Buffer A (5X)
Assay Diluent A (5X)
NUNC Maxisorp™ 96 MicroWell Plates

Cell Sources: Dendritic cells, monocytes/macrophages, B cells, T cells
Gene ID: 3592View all products for this Gene ID3593View all products for this Gene ID
UniProt: View information about IL-12 p70 on UniProt.org