Human IL-1b ELISA MAX™ Deluxe 5 Plates
ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation
BioLegend’s ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!
BioLegend’s ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.
Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.
ELISA Max™ Set Deluxe includes pre-titrated capture antibody, pre-titrated biotinylated detection antibody, pre-titrated Avidin-HRP, conveniently-sized assay calibration standards, easy-to-follow instructions, as well as, assay diluent, coating buffer, TMB substrate, and even high-affinity binding ELISA plates. Complete and ready to use!
- Reactivity
- Human
- Application
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Application Notes
BioLegend’s mouse and human IL-1β ELISA kits use antibodies raised against the mature form of IL-1β. However, because pro-IL-1β contains the mature form, these antibodies likely recognize both the pro- and the mature forms of the protein. The human IL-1β ELISA kits have been shown to equally recognize both pro- and mature forms of IL-1β.
- Application References
(PubMed link indicates BioLegend citation)
- Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
- Fuchs T, et al. 2016. Immunol Lett. 173:21-25. PubMed
- Ireland D, et al. 2016. Innate Immunity. 10.1177/1753425916672313. PubMed
- Niemiec M, et al. 2017. BMC Genomics. 10.1186/s12864-017-4097-4. PubMed
- Sensitivity
- 0.5 pg/mL
- Standard Range
- 2.0-125 pg/mL
- Materials Not Included
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
- Deionized (DI) water
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
- Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH 7.4). BioLegend Cat. No. 421601 is recommended.
- Wash bottle or automated microplate washer
- Stop Solution (2N H2SO4)
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Plate Shaker
- Plate Sealer
- Absorbent paper
- Kit Contents
- Human IL-1β ELISA MAX™ Capture Antibody (200X)
- Human IL-1β ELISA MAX™ Detection Antibody (200X)
- Human IL-1β Standard Avidin-HRP (1000X)
- Substrate Solution F
- Coating Buffer A (5X)
- Assay Diluent A (5X)
- Assay Buffer D
- NUNC Maxisorp™ 96 MicroWell Plates
- Cell Sources
- Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
- Gene ID
- NA
- UniProt
- View information about IL-1beta on UniProt.org
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