HYDRAGEL 11 VON WILLEBRAND MULTIMERS
SIMPLIFIED, SAME-DAY RESULTS, VON WILLEBRAND MULTIMERS GEL ASSAY
TEST PRESENTATION
HYDRAGEL von WILLEBRAND MULTIMERS kit is designed for the detection of abnormalities in the VWF multimers distribution related to a hemorrhagic diathesis.
The test allows to assess the overall size distribution of von Willebrand factor multimers in human plasma using agarose gel electrophoresis and immunofixation, with the semi-automated the HYDRASYS 2 instrument.
HYDRAGEL von WILLEBRAND MULTIMERS assay provides within-day results, thanks to its simplified procedure, reduced hands-on time, ready-to-use reagents, and clear and reproducible profiles.
TEST PRINCIPLE
HYDRAGEL von WILLEBRAND MULTIMERS allows separating small, intermediate and large multimers. The assay is carried out in two stages:
- Sample pre-treatment followed by an electrophoresis done in SDS agarose gel to separate proteins according to their molecular weight,
- direct immunofixation of multimers in the gel using anti-human vWF antibody followed by immunoenzyme staining to visualize the different Multimers.
The semi-automated HYDRASYS 2 instrument performs all the steps needed to obtain gels ready for visual and densitometry-based interpretations.
PERFORMANCES
- Same-day results
- Ready-to-use gels and reagents
- Excellent reproducibility
- Reduced hands-on time
- All multimer sizes
- Densitometry helps for results interpretation
- Patient’s profile overlayed with a normal control
KIT DESCRIPTION
HYDRAGEL 11 von WILLEBRAND MULTIMERS kit (PN 4360): 10 gels of 11 tests to be associated with 2 revelation kits (PN 4747)
Kit content:
- Agarose gels
- Buffered strips
- Sample diluent
- Wash solution
- Intermediate wash solution
- Rehydrating solution
- Plastic masks
- Thin filter Papers
- Thick filter Papers
5 von WILLEBRAND MULTIMERS VISUALIZATION kit (PN 4747): 2 kit are necessary for 10 gels of 11 tests associated to electrophoresis kit (PN 4360)
Kit content:
- Antiserum diluent
- Anti-von Willebrand factor antiserum
- Anti-Ig G – PER antibody
- TTF1 / TTF2 solvent
- TTF1 (stock solution)
- TTF2 (stock solution)
Related products and accessories not provided in the kit:
- HYDRAGEL 11 VON WILLEBRAND MULTIMERS ACCESSORIES SEBIA (PN 1653)
- METAL WEIGHT 2.3 KG SEBIA (PN 1654)
COMPATIBLE INSTRUMENTS
HYDRASYS 2
HYDRASYS 2 SCAN
HYDRASYS 2 SCAN FOCUSING
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