LEGEND MAX™ Human FGF-basic ELISA Kit with Pre-coated Plates 1 Pre-coated Plate
Fibroblast growth factor-basic (FGF-basic) is a heparin-binding growth factor which belongs to a family of fibroblast growth factors (FGFs). FGF family members share 35-60% amino acid sequence homology and are highly conserved among species. FGF-basic has been isolated from neural, pituitary, adrenal cortical, and placental tissues. Human FGF-basic is a 17.2 kD protein containing 154 amino acids. Other isofoms (22, 22.5, 24 and 34 kD) have also been reported with distinct intracellular localizations and functions. FGFs are involved in many physiological and pathological processes including embryonic development, neuronal outgrowth, angiogenesis, and malignant transformation. FGF-basic is essential for the development and maintenance of vascular integrity during embryogenesis and is also a major angiogenic factor involved in wound healing, cardiovascular disease, and tumor neovascularization.
BioLegend’s LEGEND MAX™ Human FGF-basic ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a human FGF-basic specific monoclonal antibody is pre-coated on a 96-well strip-well plate.
BioLegend’s LEGEND MAX™ Human FGF-basic ELISA Kit is specifically designed for the accurate quantitation of human FGF-basic from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
- Reactivity
- Human
- Application
ELISA
- Sensitivity
- 4 pg/mL
- Standard Range
- 12.5 – 800 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Human FGF-basic Pre-coated 96-well Strip Microplate
- Human FGF-basic Dectection Antibody
- Human FGF-basic Standard
- Matrix A
- Avidin-HRP E
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Cell Sources
- Brain, retina, pituitary, kidney, placenta, testis, corpus luteum, adrenal glands, monocytes, prostate, bone, liver, cartilage, endothelial cells, epithelial cells
- Gene ID
- 2247View all products for this Gene ID
- UniProt
- View information about FGF-basic on UniProt.org
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