LEGEND MAX™ Human IL-33 ELISA Kit with Pre-coated Plates 5 Pre-coated Plates | Medical Supply Company

LEGEND MAX™ Human IL-33 ELISA Kit with Pre-coated Plates 1 Pre-coated Plate

SKU: 435907 Categories: ELISA, Human, Pre-Coated, Sandwich Tag: Biolegend BV

Description
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LEGEND MAX™ Human IL-33 ELISA Kit with Pre-coated Plates 1 Pre-coated Plate

IL-33 belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1b. IL-33, IL-1β, and IL-18 are synthesized as biologically inactive precursor and are cleft by the enzyme caspase-1 to be secreted as active mature forms. IL-33 stimulates target cells by binding to the IL-1R/TLR superfamily member ST2 and subsequently activates NF-κB and MAPK pathways via identical signaling events to those observed for IL-1β. In addition, IL-33 as a nuclear factor (NF-HEV) abundantly expressed in high endothelial venules from lymphoid organs that associates with chromatin and exhibits transcriptional regulatory properties.

BioLegend’s LEGEND MAX™ Human IL-33 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IL-33 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Human IL-33 ELISA Kit is specifically designed for the accurate quantitation of Human IL-33 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Reactivity

Human

Application

ELISA

Application References (PubMed link indicates BioLegend citation)

Hu WT, et al. 2014. Mol Hum Reprod. PubMed
MA H, et al. 2012. PLoS One. 7:e29443. PubMed
Hu W, et al. 2014. Mol Hum Reprod. 20:358. PubMed
Yang Y, et al. 2016. Nat Commun. 7:11385. PubMed

Sensitivity

4.14 pg/mL

Standard Range

15.6-1,000 pg/mL

Materials Not Included

Microplate reader able to measure absorbance at 450 nm
Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
Deionized water
Wash bottle or automated microplate washer
Log-Log graph paper or software for data analysis
Tubes to prepare standard dilutions
Timer
Plate Shaker
Polypropylene vials

Kit Contents

Anti-Human IL-33 Pre-coated 96-well Strip Microplate
Human IL-33 Dectection Antibody
Human IL-33 Standard
Avidin-HRP B
Assay Buffer B
Wash Buffer (20X)
Substrate Solution F
Stop Solution
Plate Sealers

Cell Sources: Full length IL-33 localizes to the nucleus in HUVECs and transfectants C-terminal fragment, corresponding mature IL-33 is secreted.
Gene ID: 90865View all products for this Gene ID
UniProt: View information about IL-33 on UniProt.org