LEGEND MAX™ Human IL-4 ELISA Kit with Pre-coated Plates 5 Pre-coated Plates
Human IL-4 (Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1,BSF-1, Hodgkin’s cell growth factor, HCGF, Mast cell growth factor-2, MCGF-2, Macrophage fusion factor, MFF, T cell growth factor-2, TCGF-2) is a pleiotropic cytokine that is produced by activated T cells, mast cells and basophils. IL-4 elicits many different biological responses, but has two dominant functions. IL-4 regulates differentiation of naïve CD4+ T cell to Th2 cells. Th2 cells produce IL-4, IL-5, IL-10 and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. In addition, IL-4 regulates IgE and IgG1 production by B cells.
BioLegend’s LEGEND MAX™ Human IL-4 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IL-4 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.
BioLegend’s LEGEND MAX™ Human IL-4 ELISA Kit is specifically designed for the accurate quantitation of Human IL-4 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
- Reactivity
- Human
- Application
ELISA
- Sensitivity
- 0.6 pg/mL
- Standard Range
- 3.2-200 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Human IL-4 Pre-coated 96-well Strip Microplate
- Human IL-4 Dectection Antibody
- Human IL-4 Standard
- Matrix C (for serum and plasma samples only)
- Avidin-HRP B
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Cell Sources
- Mast cells, T cells, bone marrow stromal cells
- Gene ID
- 3565View all products for this Gene ID
- UniProt
- View information about IL-4 on UniProt.org
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