LEGEND MAX™ Human IL-8 ELISA Kit with Pre-coated Plates 1 Pre-coated Plate
Human IL-8 (CXCL8, neutrophil chemotactic factor, neutrophil activating protein, monocytes derived neutrophil chemotactic factor) is a member of the alpha (C-X-C) subfamily of chemokines. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation leading to local inflammation in damaged or infected tissues.
BioLegend’s LEGEND MAX™ Human IL-8 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IL-8 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.
BioLegend’s LEGEND MAX™ Human IL-8 ELISA Kit is specifically designed for the accurate quantitation of Human IL-8 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
- Reactivity
- Human
- Application
ELISA
- Application References
(PubMed link indicates BioLegend citation)
- Shi JX, et al. 2012. Am J Physiol Cell Mol Physiol. 302:793. PubMed
- Kummaraputugu AB, et al. 2013. J. Biol Chem. 288:4681. PubMed
- Shi JX, et al. 2014. Cell Signal. 26:2390. PubMed
- Shi J, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L793. PubMed
- Kummarapurugu A, et al. 2013. J Biol Chem. 288:4861. PubMed
- Shi J, et al. 2014. Int Immunopharmacol. 22:480. PubMed
- Shi J, et al. 2014. Cell Signal. 26:2390. PubMed
- Sensitivity
- 4.4 pg/mL
- Standard Range
- 15.6-1,000 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Human IL-8 Pre-coated 96-well Strip Microplate
- Human IL-8 Dectection Antibody
- Human IL-8 Standard
- Matrix A (for serum and plasma samples only)
- Avidin-HRP A
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Cell Sources
- Monocytes, lymphocytes, granulocytes, fibroblasts, endothelial cells, bronchial epithelial cells, keratinocytes, melanocytes, hepatocytes, mesangial cells, chondrocytes
- Gene ID
- 3576View all products for this Gene ID
- UniProt
- View information about IL-8 on UniProt.org
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