Soil DNA Purification Mini Kit – 50 Prep
Bead Mill Soil DNA Purification Mini Kit – 50 Prep
The Omni Soil DNA Purification Kit contains silica based spin-capture columns and nontoxic reagents. The optimized reagents are designed specifically for microbial genomic DNA extraction from soil and sediments. After sample lysis using the Omni pre-filled bead beating tubes, microbial DNA is separated from PCR inhibitors such as humic compounds. Microbial DNA is then purified through spin-column capture. Genomic DNA purified using the Omni Soil DNA Kit is ready for downstream applications such as PCR, southern blots and restriction enzyme digestion.
Product Features:
- Complete DNA purification kit including spin-columns and nontoxic reagents
- Effective removal of PCR inhibitors including humic compounds
- Microbial DNA isolation from a variety of organisms including, gram-positive and gram-negative bacteria
- Isolation of pure DNA from up to 250 mg soil, clay, sediment and compost
- Rapid sample disruption with 0.7 mm garnet beads
- Recover up to 25 ug DNA per filter
Illustrated Protocol
Ordering Information: Unit includes 50 spin-columns, 50 pre-filled bead tubes with 0.7 mm garnet beads, reagents suitable for 50 purifications and instruction guide
- DNA types: Microbial genomic DNA
- DNA purity: High quality genomic DNA with A260/A280 > 1.8
- Inhibitor removal: DNA is separated from humic compounds and other PCR inhibitors prior to purification
- DNA yield: Up to 25 ug per filter
- Capacity: Capable of microbial DNA extraction from up to 250 mg of soil and sediment
- Lysis method: Pre-filled 0.7 mm garnet bead tubes on a bead mill homogenizer or vortex mixer
- Equipment required: Bead mill or vortexer, microcentrifuge, 1.5 mL microcentrifuge tubes, water bath, heat block, 100% ethanol and isopropanol
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